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recombinant human bmp2 protein r d systems  (R&D Systems)


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    R&D Systems recombinant human bmp2 protein r d systems
    Recombinant Human Bmp2 Protein R D Systems, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 218 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+bmp2/Recombinant+Human+BMP-2+GMP+Protein%2C+CF/pm41932341-856-64-68
    Average 95 stars, based on 218 article reviews
    recombinant human bmp2 protein r d systems - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Cell Culture:

    Article Title: The role of lysophosphatidylcholine acyltransferase 2 in osteoblastic differentiation of C2C12 cells
    Article Snippet: .. To induce osteoblastic differentiation [ ], C2C12 cells were plated in 6‐well tissue culture plates at a density of 2.0 × 10 5 cells per well and cultured in differentiation medium containing DMEM with 300 ng·mL −1 human recombinant BMP2 (R&D Systems, Minneapolis, MN, USA). .. Total RNA was extracted from the cells using the RNeasy Mini Kit (QIAGEN, Valencia, CA, USA) according to the manufacturer's protocol.

    Article Title: The role of lysophosphatidylcholine acyltransferase 2 in osteoblastic differentiation of C2C12 cells.
    Article Snippet: .. To induce osteoblastic differentiation [20], C2C12 cells were plated in 6-well tissue culture plates at a density of 2.0 9 105 cells per well and cultured in differentiation medium containing DMEM with 300 ng mL 1 human recombinant BMP2 (R&D Systems, Minneapolis, MN, USA). .. Total RNA was extracted from the cells using the RNeasy Mini Kit (QIAGEN, Valencia, CA, USA) according to the manufacturer’s protocol.

    Recombinant:

    Article Title: The role of lysophosphatidylcholine acyltransferase 2 in osteoblastic differentiation of C2C12 cells
    Article Snippet: .. To induce osteoblastic differentiation [ ], C2C12 cells were plated in 6‐well tissue culture plates at a density of 2.0 × 10 5 cells per well and cultured in differentiation medium containing DMEM with 300 ng·mL −1 human recombinant BMP2 (R&D Systems, Minneapolis, MN, USA). .. Total RNA was extracted from the cells using the RNeasy Mini Kit (QIAGEN, Valencia, CA, USA) according to the manufacturer's protocol.

    Article Title: IRX-related homeobox gene MKX is a novel oncogene in acute myeloid leukemia
    Article Snippet: We used RNAeasy for RNA extraction (Qiagen), and Bioanalyzer for quality control (Agilent Technologies, Santa Clara, USA). .. Additional cell treatments were performed using 20 ng/ml recombinant BMP2 or TNFSF11 (R & D Systems, Wiesbaden, Germany), 100 μM azacytidine (AZA), 10 μM dorsomorphin, 14 μM NFkB-inhibitor, and 100 μM etoposide (Sigma-Aldrich, Taufkirchen, Germany) for 20 h. For functional testing, treated cells were analyzed using the IncuCyte S3 Live-Cell Imaging Analysis System and the Cell-by-Cell analysis software (Sartorius, Göttingen, Germany). .. For detection of apoptotic cells, we additionally used the IncuCyte Caspase-3/7 Green Apoptosis Assay diluted at 1:2000 (Sartorius).

    Article Title: The role of lysophosphatidylcholine acyltransferase 2 in osteoblastic differentiation of C2C12 cells.
    Article Snippet: .. To induce osteoblastic differentiation [20], C2C12 cells were plated in 6-well tissue culture plates at a density of 2.0 9 105 cells per well and cultured in differentiation medium containing DMEM with 300 ng mL 1 human recombinant BMP2 (R&D Systems, Minneapolis, MN, USA). .. Total RNA was extracted from the cells using the RNeasy Mini Kit (QIAGEN, Valencia, CA, USA) according to the manufacturer’s protocol.

    Article Title: Novel Indolyl-Benzimidazole Compounds Promote in vitro Wound Healing and Osteogenic Differentiation of Pluripotent Cells.
    Article Snippet: SuperSignalTM West Femto HRP Substrate solutions for chemiluminescent imaging was from Thermo Scientific (Rockford, IL, USA). .. Recombinant BMP2 was purchased from R&D Systems (Minneapolis, MN, USA). .. The RealTimeGloTM MT Cell Viability Assay Kit was purchased from Promega Corporation (Madison, WI, USA).

    Article Title: Expression of the non-coding RNA nc886 facilitates the development of tyrosine kinase inhibitor resistance in EGFR-mutated non-small-cell lung cancer cells.
    Article Snippet: .. To investigate the effect of EMT-inducing agents on the nc886 expression HCC827 cells were treated with either recombinant BMP2 (R&D systems, 355-BM) as described [39]; TGF-β1 (R&D systems, V.N.V. .. Bui et al. Biochemical and Biophysical Research Communications 731 (2024) 150395 7754-BH-005) dissolved in 4 nM HCL and 0.1 % bovine serum albumin (BSA) (Sigma-Aldrich, A2153) in concentration 10 ng/ml for 72 h; or StemXvivo EMT inducing media supplement (R&D systems, CCM017) added to the cells to reach a 100-fold dilution.

    Article Title: IRX-related homeobox gene MKX is a novel oncogene in acute myeloid leukemia.
    Article Snippet: We used RNAeasy for RNA extrac- tion (Qiagen), and Bioanalyzer for quality control (Agilent Technologies, Santa Clara, USA). .. Additional cell treatments were performed using 20 ng/ml recombinant BMP2 or TNFSF11 (R & D Systems, Wiesbaden, Germany), 100 μM azacytidine (AZA), 10 μM dorsomorphin, 14 μM NFkB-inhibitor, and 100 μM etoposide (Sigma-Aldrich, Taufkirchen, Germany) for 20 h. For functional testing, treated cells were analyzed using the IncuCyte S3 Live-Cell Imaging Analysis System and the Cell-by-Cell analysis software (Sartorius, Göttingen, Germany). .. For detection of apoptotic cells, we additionally used the IncuCyte Caspase-3/7 Green Apoptosis Assay diluted at 1:2000 (Sartorius).

    Article Title: BMP9 knockout impairs pulmonary vessel muscularisation and confers aberrant tamoxifen sensitivity.
    Article Snippet: .. Where indicated cells were treated with human recombinant BMP2, BMP9 or BMP10 (All R&D Systems). ..

    Article Title: BMP9 knockout impairs pulmonary vessel muscularisation and confers aberrant tamoxifen sensitivity
    Article Snippet: .. Where indicated cells were treated with human recombinant BMP2, BMP9 or BMP10 (All R&D Systems). ..

    Functional Assay:

    Article Title: IRX-related homeobox gene MKX is a novel oncogene in acute myeloid leukemia
    Article Snippet: We used RNAeasy for RNA extraction (Qiagen), and Bioanalyzer for quality control (Agilent Technologies, Santa Clara, USA). .. Additional cell treatments were performed using 20 ng/ml recombinant BMP2 or TNFSF11 (R & D Systems, Wiesbaden, Germany), 100 μM azacytidine (AZA), 10 μM dorsomorphin, 14 μM NFkB-inhibitor, and 100 μM etoposide (Sigma-Aldrich, Taufkirchen, Germany) for 20 h. For functional testing, treated cells were analyzed using the IncuCyte S3 Live-Cell Imaging Analysis System and the Cell-by-Cell analysis software (Sartorius, Göttingen, Germany). .. For detection of apoptotic cells, we additionally used the IncuCyte Caspase-3/7 Green Apoptosis Assay diluted at 1:2000 (Sartorius).

    Article Title: IRX-related homeobox gene MKX is a novel oncogene in acute myeloid leukemia.
    Article Snippet: We used RNAeasy for RNA extrac- tion (Qiagen), and Bioanalyzer for quality control (Agilent Technologies, Santa Clara, USA). .. Additional cell treatments were performed using 20 ng/ml recombinant BMP2 or TNFSF11 (R & D Systems, Wiesbaden, Germany), 100 μM azacytidine (AZA), 10 μM dorsomorphin, 14 μM NFkB-inhibitor, and 100 μM etoposide (Sigma-Aldrich, Taufkirchen, Germany) for 20 h. For functional testing, treated cells were analyzed using the IncuCyte S3 Live-Cell Imaging Analysis System and the Cell-by-Cell analysis software (Sartorius, Göttingen, Germany). .. For detection of apoptotic cells, we additionally used the IncuCyte Caspase-3/7 Green Apoptosis Assay diluted at 1:2000 (Sartorius).

    Live Cell Imaging:

    Article Title: IRX-related homeobox gene MKX is a novel oncogene in acute myeloid leukemia
    Article Snippet: We used RNAeasy for RNA extraction (Qiagen), and Bioanalyzer for quality control (Agilent Technologies, Santa Clara, USA). .. Additional cell treatments were performed using 20 ng/ml recombinant BMP2 or TNFSF11 (R & D Systems, Wiesbaden, Germany), 100 μM azacytidine (AZA), 10 μM dorsomorphin, 14 μM NFkB-inhibitor, and 100 μM etoposide (Sigma-Aldrich, Taufkirchen, Germany) for 20 h. For functional testing, treated cells were analyzed using the IncuCyte S3 Live-Cell Imaging Analysis System and the Cell-by-Cell analysis software (Sartorius, Göttingen, Germany). .. For detection of apoptotic cells, we additionally used the IncuCyte Caspase-3/7 Green Apoptosis Assay diluted at 1:2000 (Sartorius).

    Article Title: IRX-related homeobox gene MKX is a novel oncogene in acute myeloid leukemia.
    Article Snippet: We used RNAeasy for RNA extrac- tion (Qiagen), and Bioanalyzer for quality control (Agilent Technologies, Santa Clara, USA). .. Additional cell treatments were performed using 20 ng/ml recombinant BMP2 or TNFSF11 (R & D Systems, Wiesbaden, Germany), 100 μM azacytidine (AZA), 10 μM dorsomorphin, 14 μM NFkB-inhibitor, and 100 μM etoposide (Sigma-Aldrich, Taufkirchen, Germany) for 20 h. For functional testing, treated cells were analyzed using the IncuCyte S3 Live-Cell Imaging Analysis System and the Cell-by-Cell analysis software (Sartorius, Göttingen, Germany). .. For detection of apoptotic cells, we additionally used the IncuCyte Caspase-3/7 Green Apoptosis Assay diluted at 1:2000 (Sartorius).

    Software:

    Article Title: IRX-related homeobox gene MKX is a novel oncogene in acute myeloid leukemia
    Article Snippet: We used RNAeasy for RNA extraction (Qiagen), and Bioanalyzer for quality control (Agilent Technologies, Santa Clara, USA). .. Additional cell treatments were performed using 20 ng/ml recombinant BMP2 or TNFSF11 (R & D Systems, Wiesbaden, Germany), 100 μM azacytidine (AZA), 10 μM dorsomorphin, 14 μM NFkB-inhibitor, and 100 μM etoposide (Sigma-Aldrich, Taufkirchen, Germany) for 20 h. For functional testing, treated cells were analyzed using the IncuCyte S3 Live-Cell Imaging Analysis System and the Cell-by-Cell analysis software (Sartorius, Göttingen, Germany). .. For detection of apoptotic cells, we additionally used the IncuCyte Caspase-3/7 Green Apoptosis Assay diluted at 1:2000 (Sartorius).

    Article Title: IRX-related homeobox gene MKX is a novel oncogene in acute myeloid leukemia.
    Article Snippet: We used RNAeasy for RNA extrac- tion (Qiagen), and Bioanalyzer for quality control (Agilent Technologies, Santa Clara, USA). .. Additional cell treatments were performed using 20 ng/ml recombinant BMP2 or TNFSF11 (R & D Systems, Wiesbaden, Germany), 100 μM azacytidine (AZA), 10 μM dorsomorphin, 14 μM NFkB-inhibitor, and 100 μM etoposide (Sigma-Aldrich, Taufkirchen, Germany) for 20 h. For functional testing, treated cells were analyzed using the IncuCyte S3 Live-Cell Imaging Analysis System and the Cell-by-Cell analysis software (Sartorius, Göttingen, Germany). .. For detection of apoptotic cells, we additionally used the IncuCyte Caspase-3/7 Green Apoptosis Assay diluted at 1:2000 (Sartorius).

    Expressing:

    Article Title: Expression of the non-coding RNA nc886 facilitates the development of tyrosine kinase inhibitor resistance in EGFR-mutated non-small-cell lung cancer cells.
    Article Snippet: .. To investigate the effect of EMT-inducing agents on the nc886 expression HCC827 cells were treated with either recombinant BMP2 (R&D systems, 355-BM) as described [39]; TGF-β1 (R&D systems, V.N.V. .. Bui et al. Biochemical and Biophysical Research Communications 731 (2024) 150395 7754-BH-005) dissolved in 4 nM HCL and 0.1 % bovine serum albumin (BSA) (Sigma-Aldrich, A2153) in concentration 10 ng/ml for 72 h; or StemXvivo EMT inducing media supplement (R&D systems, CCM017) added to the cells to reach a 100-fold dilution.



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    Proteintech bmp2
    In vitro osteogenic capacity of POC-G/HA bone adhesives. (A) ALP staining and (C) ARS staining of rBMSCs cultured with POC-G/HA adhesives. Quantitative analysis of ALP activity (B) and ARS staining (D). RT-qPCR results of ALP (E), Runx-2 (F), and <t>BMP2</t> (G) for detecting relative osteogenic genes expression after 14 days incubation. Results were normalized with GAPDH. (n = 3). Western blot analysis of ALP (H), Runx-2 (I) and BMP2 (J) for detecting osteogenic-related proteins expression (n = 3). Statistical significance: ∗p < 0.05, ∗∗p < 0.001, ∗∗∗p < 0.001 (ANOVA).
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    Multiplex orthogonal gene regulation and programmable editing by OREC with therapeutic application in osteoblasts (A) Experimental illustration of orthogonal activation and repression of ASCL1 and RAB7A gene expression in HEK293T cells using distinct chemical and light inductions. (B) RT-qPCR time course analysis demonstrating orthogonal and reversible control of ASCL1 activation and RAB7A repression in response to alternating Dox and light stimuli. Data represent mean ± SEM ( n = 3). vs. RAB7A baseline (∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001); vs. RAB7A 36 h ( $ p < 0.05, $$$ p < 0.001); vs. ASCL1 baseline ( && p < 0.01, &&&& p < 0.0001); vs. ASCL1 48 h ( ## p < 0.01, #### p < 0.0001). (C) Schematic diagram illustrating crRNA guide-length-dependent transcriptional repression and HDR-based gene editing using OREC c system. (D) Transcriptional repression of RAB7A and FZD1 genes following 72 h doxycycline treatment using 15 bp crRNA guides. Data represent mean ± SEM ( n = 3) (∗∗∗∗ p < 0.0001). (E) Luciferase activity analysis following HDR-mediated luciferase knockin using 20 bp crRNA guides. Data represent mean ± SEM ( n = 3) (∗∗∗∗ p < 0.0001). (F) Flow cytometry analysis showing percentage of GFP-positive cells following HDR-mediated GFP knockin. Data represent mean ± SEM ( n = 3) (∗∗∗∗ p < 0.0001). (G) Schematic representation illustrating simultaneous orthogonal activation of Bmp2 and repression of Dkk1 expression in MC3T3-E1 cells. (H) RT-qPCR analysis demonstrating significantly enhanced Bmp2 activation and Dkk1 repression under simultaneous orthogonal induction compared with single-gene regulatory conditions. Data are presented as mean ± SEM ( n = 3). ∗ p < 0.05, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001. (I) Representative images of alizarin red S (ARS) and alkaline phosphatase (ALP) staining showing significantly enhanced osteogenic differentiation following orthogonal OREC induction compared to single-gene controls. Color code for experimental conditions: white: empty vector control; gray: OREC o construct only; crosshatched: OREC c construct only; red: OREC o + Light (optogenetic activation); green: OREC c + Dox (chemogenetic activation) (∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001). (J) RT-qPCR analysis demonstrating synergistic upregulation of osteogenic marker genes ( Alp , Bglap , and Sp7 ) upon simultaneous Bmp2 activation and Dkk1 repression. Data represent mean ± SEM ( n = 3) (∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001). (K) The CCK-8 assay quantifies viable cells via dehydrogenase-mediated formazan formation measured at 450 nm, showing that OREC treatments do not affect cell viability in MC3T3-E1 cells. Data shown as mean ± SEM ( n = 3). See also and .

    Journal: Cell Reports Methods

    Article Title: An orthogonal CRISPR/Cpf1 platform for precise spatiotemporal gene regulation and osteoporotic fracture repair

    doi: 10.1016/j.crmeth.2025.101299

    Figure Lengend Snippet: Multiplex orthogonal gene regulation and programmable editing by OREC with therapeutic application in osteoblasts (A) Experimental illustration of orthogonal activation and repression of ASCL1 and RAB7A gene expression in HEK293T cells using distinct chemical and light inductions. (B) RT-qPCR time course analysis demonstrating orthogonal and reversible control of ASCL1 activation and RAB7A repression in response to alternating Dox and light stimuli. Data represent mean ± SEM ( n = 3). vs. RAB7A baseline (∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001); vs. RAB7A 36 h ( $ p < 0.05, $$$ p < 0.001); vs. ASCL1 baseline ( && p < 0.01, &&&& p < 0.0001); vs. ASCL1 48 h ( ## p < 0.01, #### p < 0.0001). (C) Schematic diagram illustrating crRNA guide-length-dependent transcriptional repression and HDR-based gene editing using OREC c system. (D) Transcriptional repression of RAB7A and FZD1 genes following 72 h doxycycline treatment using 15 bp crRNA guides. Data represent mean ± SEM ( n = 3) (∗∗∗∗ p < 0.0001). (E) Luciferase activity analysis following HDR-mediated luciferase knockin using 20 bp crRNA guides. Data represent mean ± SEM ( n = 3) (∗∗∗∗ p < 0.0001). (F) Flow cytometry analysis showing percentage of GFP-positive cells following HDR-mediated GFP knockin. Data represent mean ± SEM ( n = 3) (∗∗∗∗ p < 0.0001). (G) Schematic representation illustrating simultaneous orthogonal activation of Bmp2 and repression of Dkk1 expression in MC3T3-E1 cells. (H) RT-qPCR analysis demonstrating significantly enhanced Bmp2 activation and Dkk1 repression under simultaneous orthogonal induction compared with single-gene regulatory conditions. Data are presented as mean ± SEM ( n = 3). ∗ p < 0.05, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001. (I) Representative images of alizarin red S (ARS) and alkaline phosphatase (ALP) staining showing significantly enhanced osteogenic differentiation following orthogonal OREC induction compared to single-gene controls. Color code for experimental conditions: white: empty vector control; gray: OREC o construct only; crosshatched: OREC c construct only; red: OREC o + Light (optogenetic activation); green: OREC c + Dox (chemogenetic activation) (∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001). (J) RT-qPCR analysis demonstrating synergistic upregulation of osteogenic marker genes ( Alp , Bglap , and Sp7 ) upon simultaneous Bmp2 activation and Dkk1 repression. Data represent mean ± SEM ( n = 3) (∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001). (K) The CCK-8 assay quantifies viable cells via dehydrogenase-mediated formazan formation measured at 450 nm, showing that OREC treatments do not affect cell viability in MC3T3-E1 cells. Data shown as mean ± SEM ( n = 3). See also and .

    Article Snippet: Recombinant BMP2 protein , MedChemExpress , Cat# HY-P7006.

    Techniques: Multiplex Assay, Activation Assay, Gene Expression, Quantitative RT-PCR, Control, Luciferase, Activity Assay, Knock-In, Flow Cytometry, Expressing, Staining, Plasmid Preparation, Construct, Marker, CCK-8 Assay

    AAV-delivered OREC system design and in vivo orthogonal gene regulation in fracture healing (A) OREC o system comprises two vectors encoding dAsCpf1-Activer (upper) and TetR-CIBN-P2A-CRY2-VP16 3 with Bmp2 -targeting crRNA (lower). (B) OREC c system consists of vectors encoding dLbCpf1-Repr (upper) and another vector expressing rtTA-Advanced with Dkk1 -targeting crRNA (lower). See also . All constructs designed within AAV packaging constraints. (C) Experimental setup showing AAV intratibial injection procedure with custom LED patch device and power supply system for localized light delivery. (D) Experimental timeline: AAV intratibial injection 14 days before fracture (day 14), tibial fracture induction (day 0), and blue light treatment (470 nm, twice daily for 30 min) beginning one day post-fracture (day 1) through endpoint (day 42). (E and F) AAV2/9 vector constructs for bioluminescence analysis of OREC activity in vivo . (G) Experimental timeline showing AAV intratibial injection (day 0) followed by 14-day treatment with light stimulation (470 nm, 30 min twice daily) or oral doxycycline (30 mg/kg daily) prior to bioluminescence imaging. (H) Representative bioluminescence images demonstrating inducible luciferase expression at tibial sites by OREC with no detectable signal in distant tissues. (I) RT-qPCR analyses of Bmp2 and Dkk1 expression levels in orthogonally treated fracture sites at days 7 and 21 post-fracture. Data represent mean ± SEM ( n = 6 mice/group) (∗∗ p < 0.01, ∗∗∗∗ p < 0.0001). (J) Analysis of Bmp2 and Dkk1 expression relative to Gapdh using the 2 −ΔCt method in fracture callus tissue 7 days after light or doxycycline induction in OREC-transduced mice. Data represent mean ± SEM ( n = 6 mice per group). (K and L) RT-qPCR analysis of Bmp2 (K) and Dkk1 (L) expression in fracture callus tissue from mice exposed to inducers without OREC constructs. Data represent mean ± SEM ( n = 3 mice per group). See also and .

    Journal: Cell Reports Methods

    Article Title: An orthogonal CRISPR/Cpf1 platform for precise spatiotemporal gene regulation and osteoporotic fracture repair

    doi: 10.1016/j.crmeth.2025.101299

    Figure Lengend Snippet: AAV-delivered OREC system design and in vivo orthogonal gene regulation in fracture healing (A) OREC o system comprises two vectors encoding dAsCpf1-Activer (upper) and TetR-CIBN-P2A-CRY2-VP16 3 with Bmp2 -targeting crRNA (lower). (B) OREC c system consists of vectors encoding dLbCpf1-Repr (upper) and another vector expressing rtTA-Advanced with Dkk1 -targeting crRNA (lower). See also . All constructs designed within AAV packaging constraints. (C) Experimental setup showing AAV intratibial injection procedure with custom LED patch device and power supply system for localized light delivery. (D) Experimental timeline: AAV intratibial injection 14 days before fracture (day 14), tibial fracture induction (day 0), and blue light treatment (470 nm, twice daily for 30 min) beginning one day post-fracture (day 1) through endpoint (day 42). (E and F) AAV2/9 vector constructs for bioluminescence analysis of OREC activity in vivo . (G) Experimental timeline showing AAV intratibial injection (day 0) followed by 14-day treatment with light stimulation (470 nm, 30 min twice daily) or oral doxycycline (30 mg/kg daily) prior to bioluminescence imaging. (H) Representative bioluminescence images demonstrating inducible luciferase expression at tibial sites by OREC with no detectable signal in distant tissues. (I) RT-qPCR analyses of Bmp2 and Dkk1 expression levels in orthogonally treated fracture sites at days 7 and 21 post-fracture. Data represent mean ± SEM ( n = 6 mice/group) (∗∗ p < 0.01, ∗∗∗∗ p < 0.0001). (J) Analysis of Bmp2 and Dkk1 expression relative to Gapdh using the 2 −ΔCt method in fracture callus tissue 7 days after light or doxycycline induction in OREC-transduced mice. Data represent mean ± SEM ( n = 6 mice per group). (K and L) RT-qPCR analysis of Bmp2 (K) and Dkk1 (L) expression in fracture callus tissue from mice exposed to inducers without OREC constructs. Data represent mean ± SEM ( n = 3 mice per group). See also and .

    Article Snippet: Recombinant BMP2 protein , MedChemExpress , Cat# HY-P7006.

    Techniques: In Vivo, Plasmid Preparation, Expressing, Construct, Injection, Activity Assay, Imaging, Luciferase, Quantitative RT-PCR

    Comparative effects of BMP9 and BMP2 on osteogenic differentiation and osteoclastogenesis in vitro. (A) Real‐time PCR analysis of key osteogenic genes (Col1, Runx2, ALP, and OCN) in MC3T3‐E1 cells treated with 8 nM of BMP2 or BMP9 for 3, 5, and 7 days. All gene‐expression levels were normalized to GAPDH. (B) Western blot analysis of osteogenic marker proteins in cell lysates harvested after 7 days of treatment with BMP2 or BMP9. GAPDH was used as the loading control. Densitometric quantification of band intensities (integrated density) normalized to GAPDH is shown below the blots and presented as relative protein expression. (C) Western blot showing dose‐dependent p‐Smad1/5/9 in MC3T3‐E1 cells exposed to varying concentrations of BMP2 or BMP9. Phosphorylation was quantified by densitometry and expressed as fold change vs. control after normalization using [(p‐Smad1/5/9)/(total Smad1/5/9)] and further normalized to GAPDH, as shown in the graph below the blots. Asterisks indicate statistical significance for pairwise comparisons between BMP2 and BMP9 at the same concentration (****, p < 0.0001), unless otherwise indicated. (D) ALP activity and representative images of ALP staining in MC3T3‐E1 cultures after 7 days of induction with BMP2 or BMP9. (E) Alizarin Red S staining illustrating mineralized nodule formation after extended culture with BMP2 or BMP9. (F) Representative TRAP‐stained images of RAW 264.7‐derived osteoclasts following treatment with RANKL (3 nM), BMP2 (8 nM), or BMP9 (8 nM) for 5 days. TRAP‐positive multinucleated osteoclasts are indicated by arrows. Scale bar, 20 μm. (G) Quantification of TRAP‐positive multinucleated cells per well. Data are presented as the mean ± SD ( n = 3 independent experiments), and p ‐values were calculated using one‐way analysis of variance (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001). BMP, bone morphogenetic protein; PCR, polymerase chain reaction; ALP, alkaline phosphatase; Col1, collagen type I; Runx2, runt‐related transcription factor 2; OCN, osteocalcin; GAPDH, glyceraldehyde‐3‐phosphate dehydrogenase.

    Journal: Clinical Implant Dentistry and Related Research

    Article Title: Bone Morphogenetic Protein ( BMP ) 9 Outperforms BMP2 in Osteogenesis and Osseointegration: In Vitro and In Vivo

    doi: 10.1111/cid.70135

    Figure Lengend Snippet: Comparative effects of BMP9 and BMP2 on osteogenic differentiation and osteoclastogenesis in vitro. (A) Real‐time PCR analysis of key osteogenic genes (Col1, Runx2, ALP, and OCN) in MC3T3‐E1 cells treated with 8 nM of BMP2 or BMP9 for 3, 5, and 7 days. All gene‐expression levels were normalized to GAPDH. (B) Western blot analysis of osteogenic marker proteins in cell lysates harvested after 7 days of treatment with BMP2 or BMP9. GAPDH was used as the loading control. Densitometric quantification of band intensities (integrated density) normalized to GAPDH is shown below the blots and presented as relative protein expression. (C) Western blot showing dose‐dependent p‐Smad1/5/9 in MC3T3‐E1 cells exposed to varying concentrations of BMP2 or BMP9. Phosphorylation was quantified by densitometry and expressed as fold change vs. control after normalization using [(p‐Smad1/5/9)/(total Smad1/5/9)] and further normalized to GAPDH, as shown in the graph below the blots. Asterisks indicate statistical significance for pairwise comparisons between BMP2 and BMP9 at the same concentration (****, p < 0.0001), unless otherwise indicated. (D) ALP activity and representative images of ALP staining in MC3T3‐E1 cultures after 7 days of induction with BMP2 or BMP9. (E) Alizarin Red S staining illustrating mineralized nodule formation after extended culture with BMP2 or BMP9. (F) Representative TRAP‐stained images of RAW 264.7‐derived osteoclasts following treatment with RANKL (3 nM), BMP2 (8 nM), or BMP9 (8 nM) for 5 days. TRAP‐positive multinucleated osteoclasts are indicated by arrows. Scale bar, 20 μm. (G) Quantification of TRAP‐positive multinucleated cells per well. Data are presented as the mean ± SD ( n = 3 independent experiments), and p ‐values were calculated using one‐way analysis of variance (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001). BMP, bone morphogenetic protein; PCR, polymerase chain reaction; ALP, alkaline phosphatase; Col1, collagen type I; Runx2, runt‐related transcription factor 2; OCN, osteocalcin; GAPDH, glyceraldehyde‐3‐phosphate dehydrogenase.

    Article Snippet: Recombinant human BMP2 (R&D Systems, Minneapolis, MN, USA) was used as the control.

    Techniques: In Vitro, Real-time Polymerase Chain Reaction, Gene Expression, Western Blot, Marker, Control, Expressing, Phospho-proteomics, Concentration Assay, Activity Assay, Staining, Derivative Assay, Polymerase Chain Reaction

    Experimental timeline, surgical procedure, and implant stability in the beagle saddle‐type peri‐implant defect model. (A) Timeline of the in vivo study. On the surgery day a saddle‐type peri‐implant defect was created, a dental implant was inserted, and bone grafting was performed with group allocation as follows. Non‐graft, collagenated xenograft matrix only, collagenated xenograft matrix + BMP2, and collagenated xenograft matrix + BMP9. Calcein was injected subcutaneously on day 54. At 8 weeks implant stability was recorded, micro CT was acquired, and animals were sacrificed. (B) Surgical procedure. (C) ISQ measured immediately before sacrifice. Bars show mean ± SD. Asterisks indicate statistical significance as marked in the plot.

    Journal: Clinical Implant Dentistry and Related Research

    Article Title: Bone Morphogenetic Protein ( BMP ) 9 Outperforms BMP2 in Osteogenesis and Osseointegration: In Vitro and In Vivo

    doi: 10.1111/cid.70135

    Figure Lengend Snippet: Experimental timeline, surgical procedure, and implant stability in the beagle saddle‐type peri‐implant defect model. (A) Timeline of the in vivo study. On the surgery day a saddle‐type peri‐implant defect was created, a dental implant was inserted, and bone grafting was performed with group allocation as follows. Non‐graft, collagenated xenograft matrix only, collagenated xenograft matrix + BMP2, and collagenated xenograft matrix + BMP9. Calcein was injected subcutaneously on day 54. At 8 weeks implant stability was recorded, micro CT was acquired, and animals were sacrificed. (B) Surgical procedure. (C) ISQ measured immediately before sacrifice. Bars show mean ± SD. Asterisks indicate statistical significance as marked in the plot.

    Article Snippet: Recombinant human BMP2 (R&D Systems, Minneapolis, MN, USA) was used as the control.

    Techniques: In Vivo, Injection, Micro-CT

    In vitro osteogenic capacity of POC-G/HA bone adhesives. (A) ALP staining and (C) ARS staining of rBMSCs cultured with POC-G/HA adhesives. Quantitative analysis of ALP activity (B) and ARS staining (D). RT-qPCR results of ALP (E), Runx-2 (F), and BMP2 (G) for detecting relative osteogenic genes expression after 14 days incubation. Results were normalized with GAPDH. (n = 3). Western blot analysis of ALP (H), Runx-2 (I) and BMP2 (J) for detecting osteogenic-related proteins expression (n = 3). Statistical significance: ∗p < 0.05, ∗∗p < 0.001, ∗∗∗p < 0.001 (ANOVA).

    Journal: Materials Today Bio

    Article Title: Pressure-sensitive composite bandages with high toughness, self-healability and strong tissues adhesion for rapid bone fixation and accelerated osteogenesis via immunomodulation and neovascularization

    doi: 10.1016/j.mtbio.2025.102730

    Figure Lengend Snippet: In vitro osteogenic capacity of POC-G/HA bone adhesives. (A) ALP staining and (C) ARS staining of rBMSCs cultured with POC-G/HA adhesives. Quantitative analysis of ALP activity (B) and ARS staining (D). RT-qPCR results of ALP (E), Runx-2 (F), and BMP2 (G) for detecting relative osteogenic genes expression after 14 days incubation. Results were normalized with GAPDH. (n = 3). Western blot analysis of ALP (H), Runx-2 (I) and BMP2 (J) for detecting osteogenic-related proteins expression (n = 3). Statistical significance: ∗p < 0.05, ∗∗p < 0.001, ∗∗∗p < 0.001 (ANOVA).

    Article Snippet: Transcript levels of ALP , BMP2 , and Runx-2 were measured using SYBR Premix Ex Taq II (primer sequences in ), with GAPDH (Proteintech, USA) serving as the reference gene.

    Techniques: In Vitro, Staining, Cell Culture, Activity Assay, Quantitative RT-PCR, Expressing, Incubation, Western Blot